![]() If you want to reproduce the whole article If you are the author of this article, you do not need to request permission to reproduce figuresĪnd diagrams provided correct acknowledgement is given. Provided correct acknowledgement is given. If you are an author contributing to an RSC publication, you do not need to request permission To request permission to reproduce material from this article, please go to the Thiophene-based organic dye with large Stokes shift and deep red emission for live cell NAD(P)H detection under varying chemical stimuli Overall, this work presents a significant advancement in the field of live cell imaging by providing a simple and cost-effective method for detecting changes in NAD(P)H concentration under varying chemical stimuli. Notably, cisplatin treatment increased the NAD(P)H concentration in A459 cells over time. Furthermore, the probes were able to image NAD(P)H in Drosophila melanogaster larvae. They are also photostable and were successfully used to monitor changes in NADH concentration in live cells during glycolysis in the presence of glucose, lactate, and pyruvate, treatment of FCCP and cancer drug cisplatin, and under hypoxia triggered by CoCl 2. The probes are highly selective for NAD(P)H, water-soluble, biocompatible, and easily permeable to cells. Probe A has a large Stokes shift of 144 nm, which allows for better separation between the excitation and emission spectra, reducing spectral overlap and improving the accuracy of fluorescence measurements. This is due to the NADH-facilitated reduction of the 1-methylquinolinium unit into 1-methyl-1,4-dihydroquinoline, which then acts as the electron donor for the probes, leading to the formation of well-defined electron donor–acceptor dye systems. However, upon the presence of NADH, new absorption and fluorescence peaks appear at 477 nm and 619 nm for probe A and at 486 nm and 576 nm for probe B, respectively. These probes display weak absorption peaks at 315 nm ( A) and 334 nm ( B) and negligible fluorescence in the absence of NADH. The probes were prepared using thiophene-based organic dyes featuring a π-conjugated bridge of thiophene and 3,4-ethylenedioxythiophene units linking the 1-methylquinolinium acceptor and formyl acceptor, respectively. Which leaves you free to move on to your next project.We report a novel method for synthesizing red and deep red cyanine dyes with large Stokes shifts, probes A and B, for live cell NAD(P)H detection. With a double-click of the Ctrl key, FastFox will complete your thought and increase your productivity. These frequently used phrases are then suggested to you as you type. FastFox pays attention to common phrases for you and will learn the phrases that you repeatedly type. Intuitive AutocompleteYou may be wondering which phrases you use frequently throughout the day and where to begin creating shortcuts. Now you are ready to use the macro, sit back and watch the program work for you. Use a combination of Ctrl, Alt and Shift with other key strokes to create the shortcut keys for your macro, enter your command for the set of actions and save it. Fortunately, those with a technical aptitude can easily share their macros with other FastFox users. Enter the dimensions and save your shortcut.Ĭreating a MacroMacros are for the more advanced user. ![]() Enter the shortcut keys, such as 'cologo' and import the image. Have a nice day!' You will be amazed at your increased productivity and fast typing.Ĭreating a shortcut to insert your company logo sized to 110px x 150px is just as simple. Next time you want to use the phrase, just type ty and it will expand to 'Thank you. Have a nice day!" Select a keyboard shortcut such as 'ty' and save your new text shortcut. Enter a frequently used phrase, such as, "Thank you.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |